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An evaluation of the use of seizure prone rats when investigating intermediate stage sleep.

Morrissey MJ, Anch AM, Duntley SP

Department of Psychology, Saint Louis University, MO 63103, USA. morrisseym@neuro.wustl.edu

A body of literature is developing which identifies an additional stage of sleep in rats, cats and mice. Intermediate stage (IS) sleep is a measurable sleep stage that is maintained by the hyperpolarization of GABA(A) containing thalamocortical neurons. The present study attempts to clarify inconsistencies within the sleep spindle literature. Most notably, inconsistencies between those that study sleep spindles in the rat outside and within the context of IS sleep. Ten male taiep rats weighing from 400 g to 600 g, and 9-12 months of age, were used in this study. The animals were given a one-time, .9 mg/kg dose of the benzodiazepine clonazepam. The control group had more seizure activity (mean = 13.4) than the treatment group (mean = 5.2, t(1-18) = 8.859, p < .001), and had a lower number of sleep spindles (mean=10.3) than the treatment group (mean = 13.3, t(1-18) = -3.4, p < .001). In addition, spectral analysis of sleep spindles during IS and seizure activity revealed that sleep spindles are within the frequency band of 8-11Hz, while seizure activity is within the 4-7 Hz range. This data supports the hypotheses that sleep spindles are distinguishable from seizure activity.

Published 7 April 2005 in J Neurosci Methods, 143(2): 159-62.
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